ELISPOT KITS - Autoimmun Diagnostika GmbH

About EliSpot

EliSpot (Enzyme-Linked ImmunoSpot) is the most sensitive immunoassay and allows the monitoring of secreted cytokines even at the single T-cell level. With EliSpot it is possible to detect one specific cell out of 100.000 irrelevant cells, making this method much more sensitive than traditional ELISA applications.

Measurement of antigen-specific cytokine production of single T-cells
High sensitivity, high signal- to-noise ratio
Reproducible and reliable results within 24 hours

Two-color diagnostic iSpot assays: Explore the future of Elispot diagnostics

Red spot: cell secreting IL-2 only

Green spot: cell secreting γ-INF only (effector cell)

Double-stained spot: cell secreting both, γ-INF and IL-2 (memory cell)

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AID EliSpot Assay Kits

AID offers ready to use 96-well-plates, pre-coated with primary antibody, in a complete kit containing secondary antibody, conjugate and substrate. In addition, AID offers a selection of ready to use diagnostic kits, specific antigens already included.

Assays for routine diagnostics

Parameter	Art.- No.
BasicSpot	ELSP 5500
Interferon-γ assay for detection of various specific T-cells.
LymeSpot	ELSP 5510
Interferon-γ assay for the specific detection of T-cells secreted due to a Borrelia infection.
EBV-Spot	ELSP 5520
Interferon-γ assay for detection of Epstein-Barr-Virus (EBV) specific T-cells. Combination of EBV-lytic (BMLF1, BRLF1, BZLF1, ZEBRA) and EBV-latent (EBNA3a, LMP2) peptide mixes.
CMV-Spot	ELSP 5530
Interferon-γ assay for detection of Cytomegalovirus (CMV) specific T-cells.
TransSpot	ELSP 5550
Interferon-γ assay for monitoring of transplantation patients and for detection of latent/chronic infections important in terms of a planned transplantation. For more information on the relevance of the EliSpot method in transplantation please read here.

Basic research kits in 96-well format (Customized kits available!)

Parameter	Art.- No.
Human
Interferon-γ	ELSP 5000
TNF-α	ELSP 5080
Interleukin-2	ELSP 5050
Interleukin-4	ELSP 5060
Interleukin-5	ELSP 5010
Interleukin-6	ELSP 5020
Interleukin-10	ELSP 5040
Interleukin-12	ELSP 5090
Interleukin-13	ELSP 5052
GM-CSF	ELSP 5053
Mouse
murine Interferon-γ	ELSP 5025

For information about our other diagnostic products for example
Immobilized DNA probes, Immunoblots or Immunofluorescence,
please visit our website: