About EliSpot

 

 
 

EliSpot (Enzyme-Linked Immunosorbent Spot) is the most sensitive immunoassay and allows the monitoring of secreted cytokines even at the single T-cell level. With EliSpot it is possible to detect one specific cell out of 100.000 irrelevant cells, making this method much more sensitive than traditional ELISA applications.

 

 
 
  • Measurement of antigen-specific cytokine production of single T-cells
  • High sensitivity, high signal- to-noise ratio
  • Reproducible and reliable results within 24 hours
EliSpot  
   
How EliSpot works: An animation of the procedure
EliSpot  
       
  AID EliSpot Assay Kits

 

 
 

AID offers ready to use 96-well-plates, pre-coated with primary antibody, in a complete kit containing secondary antibody, conjugate and substrate. In addition, AID offers a selection of ready to use diagnostic kits, specific antigens already included.

 
 


Basic research kits in 96-well format (Customized kits available!)

Parameter

Art.- No.

Human

 

Interferon-γ

ELSP 5000

TNF-α

ELSP 5080

Interleukin-2

ELSP 5050

Interleukin-4

ELSP 5060

Interleukin-5

ELSP 5010

Interleukin-6

ELSP 5020

Interleukin-10

ELSP 5040

Interleukin-12  

ELSP 5090

Interleukin-13

ELSP 5052

GM-CSF

ELSP 5053

Mouse

 

murine Interferon-γ

ELSP 5025

   
       
 

Assays for routine diagnostics

Parameter

Art.- No.

BasicSpot

ELSP 5500

Interferon-γ assay for detection of various specific T-cells. For a collection of antigens available at AID please click here

 

LymeSpot

ELSP 5510

Interferon-γ assay for the specific detection of T-cells secreted due to a Borrelia infection.

 

EBV-Spot

ELSP 5520

Interferon-γ assay for detection of Epstein-Barr-Virus (EBV) specific T-cells. Combination of EBV-lytic (BMLF1, BRLF1, BZLF1, ZEBRA) and EBV-latent (EBNA3a, LMP2) peptide mixes.

 

CMV-Spot

ELSP 5530

Interferon-γ assay for detection of Epstein-Barr-Virus (CMV) specific T-cells.

 

TransSpot

ELSP 5550

Interferon-γ assay for monitoring of transplantation patients and for detection of latent/chronic infections important in terms of a planned transplantation. For more information on the relevance of the EliSpot method in transplantation please read here.

 

   
 

For information about our other diagnostic products for example
Immobilized DNA probes, Immunoblots or Immunofluorescence,
please visit our website:

www.aid-diagnostika.com

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