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AID macSpot System: EliSpot meets Design
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| Apple® and iMac® are trademarks of Apple Inc., registered in the U.S. and other countries. |
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Worldwide the first and only EliSpot reader running under the control of an Apple Macintosh Sytem AID has once more reacted on users demand and has combined it’s successful iSpot reader system with Apple’s all-in-one iMac, a complete, high-performance computer in a beautifully thin design. For a limited time period only for 39.900 US$ |
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Contact our new office in USA: |
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Special offer : Buy a standard AID EliSpot Reader ELR04 HR and get the new enzymatic/fluorescent Reader iSpot for the same price!!! Low in budget? – Try out the AID EliSpot Reader ELR04-LB,
now only for 19.900 USD !!!!!!! |
Be prepared for the EliSpot future and analyse your enzymatic assays and your two-color fluorospot assays in the same innovative AID Elispot Reader.
Scanning and counting and analysing with only one software in just two minutes in the enzymatic mode. Fluorospot-count in just 6-7 minutes
- Software driven filter cube
- Only a mouse-click from enzymatic to fluorescent scanning and vice versa
- Prepared for Cy3/FITC two-color analysis, other combinations on demand
- Sophisticated new imaging technology to detect single- and double-stained spots with 100% accuracy
- FDA CFR part 11compliant, CE-certificated
- Achieves the same sensitivity as with enzymatic based assays
Why Fluorescent EliSpot?
To overcome this problem researches have begun to develop EliSpot assays based on fluorescent labeling. Detection of multiple cytokines expressing lymphocytes such as double IFNgamma-IL-2 producing T-cells with this new EliSpot (FluoroSpot)-technology could provide additional and new informations regarding the clinical outcome in patients with various viral infections and other questions.
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advanced TH1/TH2 analysis (e.g. Interferon vs. IL2 and IL10)
more detailed analysis of T cell subpopulations
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improved characterisation of shifted immune response in various infectious diseases
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IFN-assay done with traditional enzymatic (left) and with fluorescent-based EliSpot (right) and analysed with the AID EliSpot software. Sensitivity of both assays is nearly the same, as shown by the total number of spots in both wells.
Why AID iSpot?
AID has developed an unique EliSpot device capable to analyse fluorescent- as well as traditional enzymatic-based EliSpot assays.
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Since commercially available validated fluorescent-based EliSpot assays are still in development but should be on the market in 2008, it is reasonable to use a hybrid-technolgy, which allows to interprete enzymatic- and fluorescent-based assays with only one device.
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The new AID iSpot Reader is still as fast as the original AID Readers and completely interpretes a 96-well fluorescent plate within 6 –7 minutes.
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The AID iSpot Reader can switch between both approaches without hardware changes and without loss of sensitivity in the assay analysis.
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The sophisticated new overlay-technology allows for detailed interpretation of double-expressing T-cells in every well. The adapted fluorescent interpretation software especially facilitates working with these subtypes of cells.
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Simultaneously detection of multiple fluorescent dyes with universal filters or high-sensitivity detection of two fluorophores with specific filters is supported by the software.
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Fluorescent-based two-color IFN/Il2-assay interpreted with the AID EliSpot software: Although there are only two IL2 secreting cells (image A, Cy3), both spots could be found in the IFN image as well (image B, FITC) and are therefore pointed out in the overlaid image C. Note that the artefact (art.) seen in image A (Cy3) is not interpreted as a spot by the software.
On the following pages you will find information on the AID Elispot Reader Systems and the Elispot assays developed and produced by AID.
Ebinger Strasse 4
D-72479 Strassberg
Germany
Phone: +49-7434-9364-0
Fax: +49-7434-9364-40
e-mail: info(at)elispot(dot)com
For further informations on other AID
products,such as molecular diagnostic kits, immunoblots, Hep-2 screening
kits, automated Colony counters, Zone readers, Biochip readers and fully
automated microscopes please visit our other websites:
www.colony-counter.com