About EliSpot

EliSpot (Enzyme-Linked Immunosorbent Spot) is the most sensitive immunoassay and allows the monitoring of secreted cytokines even at the single T-cell level. With EliSpot it is possible to detect one specific cell out of 100.000 irrelevant cells, making this method much more sensitive than traditional ELISA applications.

  • Measurement of antigen-specific cytokine production of single T-cells
  • High sensitivity, high signal- to-noise ratio
  • Reproducible and reliable results within 24 hours

Multiple-color diagnostic iSpot assays: Explore the future of Elispot diagnostics

Red spot: cell secreting IL-2 only
Green spot: cell secreting γ-INF only (effector cell)
Double-stained spot: cell secreting both, γ-INF and IL-2 (memory cell)
 

 

AID EliSpot Assay Kits

AID offers ready to use 96-well-plates, pre-coated with capture antibodies, in a complete kit containing detection antibodies, conjugate and substrate. In addition, AID offers a selection of ready to use diagnostic kits, specific antigens already included.

Assays for routine diagnostics

 

  Art.- No.

LymeSpot

ELSP 5510

IFN-γ assay for detection of specific T-cells due to Borrelia infection.

 

EBV-Spot

ELSP 5520

IFN-γ assay for detection of Epstein-Barr-Virus (EBV) specific T-cells. Combination of EBV-lytic (BMLF1, BRLF1, BZLF1, ZEBRA) and EBV-latent (EBNA3a, LMP2) peptide mixes.

 

TB-Spot

ELSP5540

IFN-γ assay for detection of Mycobacterium tuberculosis specific T-cells in latent and active state of infection. By using antigens, which are not present in BCG-vaccination strain, no cross reactivity occurs at vaccinated persons.

 

 

 Basic research kits in 96-well format (Customized kits available!)

 

 

Art.- No.

Human

 

IFN-γ

ELSP 5000

TNF-α

ELSP 5080

Interleukin-2

ELSP 5050

Interleukin-4

ELSP 5060

Interleukin-5

ELSP 5010

Interleukin-6

ELSP 5020

Interleukin-10

ELSP 5040

Interleukin-12  

ELSP 5090

Interleukin-13

ELSP 5052

GM-CSF

ELSP 5053

iSpot kits

 

Interferon gamma + Interleukin-2

ELSP5710/5810

Interferon gamma + Interleukin-5

ELSP5720/5820

Interferon gamma + Interleukin-17

ELSP5730/5830

Interferon gamma + Interleukin-2 + Interleukin-17

ELSP6000/6100

For information about our other diagnostic products for example
Immobilized DNA probes, Immunoblots or Immunofluorescence,
please visit our website:

www.aid-diagnostika.com