EliSpot (Enzyme-Linked Immunosorbent Spot) is the most sensitive immunoassay and allows the monitoring of secreted cytokines even at the single T-cell level. With EliSpot it is possible to detect one specific cell out of 100.000 irrelevant cells, making this method much more sensitive than traditional ELISA applications.
- Measurement of antigen-specific cytokine production of single T-cells
- High sensitivity, high signal- to-noise ratio
- Reproducible and reliable results within 24 hours
Multiple-color diagnostic iSpot assays: Explore the future of Elispot diagnostics
|Red spot: cell secreting IL-2 only|
|Green spot: cell secreting γ-INF only (effector cell)|
|Double-stained spot: cell secreting both, γ-INF and IL-2 (memory cell)|
AID EliSpot Assay Kits
AID offers ready to use 96-well-plates, pre-coated with capture antibodies, in a complete kit containing detection antibodies, conjugate and substrate. In addition, AID offers a selection of ready to use diagnostic kits, specific antigens already included.
Assays for routine diagnostics
IFN-γ assay for detection of specific T-cells due to Borrelia infection.
IFN-γ assay for detection of Epstein-Barr-Virus (EBV) specific T-cells. Combination of EBV-lytic (BMLF1, BRLF1, BZLF1, ZEBRA) and EBV-latent (EBNA3a, LMP2) peptide mixes.
IFN-γ assay for detection of Mycobacterium tuberculosis specific T-cells in latent and active state of infection. By using antigens, which are not present in BCG-vaccination strain, no cross reactivity occurs at vaccinated persons.
Basic research kits in 96-well format (Customized kits available!)
Interferon gamma + Interleukin-2
Interferon gamma + Interleukin-5
Interferon gamma + Interleukin-17
Interferon gamma + Interleukin-2 + Interleukin-17
For information about our other diagnostic products for example
Immobilized DNA probes, Immunoblots or Immunofluorescence,
please visit our website: